TY - JOUR T1 - Investigation of MMP-2 and -9 in a Highly Invasive A431 Tumor Cell Sub-line Selected from a Boyden Chamber Assay JF - Anticancer Research JO - Anticancer Res SP - 2109 LP - 2120 VL - 28 IS - 4B AU - WEN-TE KAO AU - CHUN-YU LIN AU - LUNG-TA LEE AU - PING-PING H. LEE AU - CHIN-CHUN HUNG AU - YUNG-SHENG LIN AU - SHIH-HSUN CHEN AU - FERNG-CHUN KE AU - JIUAN-JIUAN HWANG AU - MING-TING LEE Y1 - 2008/07/01 UR - http://ar.iiarjournals.org/content/28/4B/2109.abstract N2 - In this study, highly invasive tumor cell lines (designated A431-I, -II and -III) derived from parental A431 tumor cells (A431-P) were isolated by three successive passages through a Boyden chamber with matrigel-coated membrane support. The invasive potential and the activity of secreted MMP-9 of each sub-line increased significantly compared to the A431-P (A431-III > A431-II > A431-I) as evidenced by the in vitro invasion assay, gelatin zymography and immunoblotting analyses. RT-PCR results also revealed the elevated expression of MMP-9 in A431-III. We further characterized the A431-III sub-line and found these cells exhibited a greater potential for attachment and spreading on fibronectin-coated substratum and for migration. The A431-III cells displayed multiple cytoplasmic extensions with focal contacts (vinculin-positive staining) during cell spreading within 30 min. We also noticed an increase in FAK phosphorylation, but no significant change in FAK protein level in the A431-III sub-line compared to those of A431-P cells. Together, these results demonstrate that the greater invasion potential exhibited by the highly invasive A431-III subline is likely attributed to an increased ability for attachment, spreading and migration, as well as increased MMP activity. Thus, A431-P and the highly invasive A431-III sub-line could be an excellent model for studying the mechanism of cancer metastasis. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -