RT Journal Article SR Electronic T1 Regulation of Inflammation- and Angiogenesis-related Gene Expression in Breast Cancer Cells and Co-cultured Macrophages JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 2093 OP 2099 VO 28 IS 4B A1 GISELLE T. BURNETT A1 DENISE C. WEATHERSBY A1 TIFFANY E. TAYLOR A1 THEODORE A. BREMNER YR 2008 UL http://ar.iiarjournals.org/content/28/4B/2093.abstract AB Background: Tumor-associated macrophages (TAMs) secrete key modifiers of tumor progression and their modification has been proposed as a therapeutic strategy. Phenotypic changes that may render TAMs selectively vulnerable to anti-cancer agents were examined. Materials and Methods: Gene arrays, reverse transcription-polymerase chain reaction and Western blotting were used to study inflammation- and angiogenesis-related gene expression in co-cultured breast cancer cells and macrophages and to determine how their interactions were affected by tamoxifen and aspirin. Results: MCF-7 (mammary adenocarcinoma) cells down-regulated macrophage migration inhibitory factor (MIF), but tamoxifen-pretreated MCF-7 cells up-regulated MIF in co-cultured macrophages. Two molecular variants of MIF were observed in the co-cultured MCF-7 cells. Aspirin induced IL-10 expression in the macrophages, MCF-7 and tamoxifen-pretreated MCF-7 cells. Aspirin-pretreated macrophages potently induced IL-10 expression in the MCF-7 cells. Conclusion: Because MIF is a determinant of the M1 macrophage activation state, the MCF-7-induced ablation of MIF in TAMs is suggestive of partial M2 polarization. Tamoxifen modulates MCF-7 regulation of TAM gene expression and aspirin alters macrophage regulation of MCF-7 gene expression. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved