PT  - JOURNAL ARTICLE
AU  - TAKAYA, YOSHIHIRO
AU  - TANAKA, KUNIHIKO
AU  - HIRAKAWA, CHIASA
AU  - TAGAWA, YUTAKA
AU  - NIWA, MASAMI
TI  - New Method for Separation of Subpopulations from a Heterogeneous Colon Cancer Cell Line
DP  - 2008 Nov 01
TA  - Anticancer Research
PG  - 3665--3670
VI  - 28
IP  - 6A
4099  - http://ar.iiarjournals.org/content/28/6A/3665.short
4100  - http://ar.iiarjournals.org/content/28/6A/3665.full
SO  - Anticancer Res2008 Nov 01; 28
AB  - Background: To understand the heterogeneity of human colon cancers, a new method to separate cancer subpopulations was developed. Materials and Methods: Cells from a human colon cancer cell line, DLD-1, were seeded on an 8 μm pore membrane. After six hours, the cells which remained beneath the membrane as well as the cells which dropped onto the 24-well plate were collected. To clarify the differences between the two subpopulations, transepithelial electrical resistance (TEER) and immunocytochemistry were evaluated. Results: Two subpopulations, clones D and A, were separated from DLD-1 with the newly developed method. Both subpopulations showed quite different TEER values and different arrangements of cell-cell contact. In addition, the distinct subcellular localizations of claudin family proteins and zonula occludens-1 (ZO-1) were identified. Conclusion: A new separation method to isolate colon cancer subpopulations was established in which the intercellular junctions differed. This method can be considered as a helpful tool in the investigation of colon cancer heterogeneity. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved