RT Journal Article
SR Electronic
T1 The Synthesis and Activity of Lipoprotein Lipase in the Subcutaneous Adipose Tissue of Patients with Musculoskeletal Sarcomas
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2081
OP 2086
VO 28
IS 4B
A1 SAKAYAMA, KENSHI
A1 KIDANI, TERUKI
A1 TANJI, NOZOMU
A1 YAMAMOTO, HARUYASU
A1 MASUNO, HIROSHI
YR 2008
UL http://ar.iiarjournals.org/content/28/4B/2081.abstract
AB The purpose of this study was to explore the triacylglycerol (TG) deposition and lipoprotein lipase (LPL) activity in the adipose tissue of patients with muculoskeletal sarcoma. Subcutaneous adipose tissue was obtained from the thighs of 19 patients with musculoskeletal sarcomas (sarcoma group) and 20 patients with osteoarthritis of the hip joint (control group) at surgery. The adipose tissue was homogenized and aliquots of the homogenate were used to measure the TG content and to prepare an acetone/ether powder to measure the LPL activity. The TG content was higher, but not significantly, in the sarcoma group than in the control group. The LPL activity of the sarcoma group was significantly higher than that of the control group. The TG content of the sarcoma group correlated positively with the LPL activity. [35S]Methionine incorporation investigation showed that the rate of LPL synthesis was significantly higher in the sarcoma group than in the control group. These results indicated that LPL was up-regulated at the transcriptional/translational level, thus resulting in an increased TG deposition in the adipose tissue of patients with muculoskeletal sarcoma. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved