PT - JOURNAL ARTICLE AU - KENSHI SAKAYAMA AU - TERUKI KIDANI AU - NOZOMU TANJI AU - HARUYASU YAMAMOTO AU - HIROSHI MASUNO TI - The Synthesis and Activity of Lipoprotein Lipase in the Subcutaneous Adipose Tissue of Patients with Musculoskeletal Sarcomas DP - 2008 Jul 01 TA - Anticancer Research PG - 2081--2086 VI - 28 IP - 4B 4099 - http://ar.iiarjournals.org/content/28/4B/2081.short 4100 - http://ar.iiarjournals.org/content/28/4B/2081.full SO - Anticancer Res2008 Jul 01; 28 AB - The purpose of this study was to explore the triacylglycerol (TG) deposition and lipoprotein lipase (LPL) activity in the adipose tissue of patients with muculoskeletal sarcoma. Subcutaneous adipose tissue was obtained from the thighs of 19 patients with musculoskeletal sarcomas (sarcoma group) and 20 patients with osteoarthritis of the hip joint (control group) at surgery. The adipose tissue was homogenized and aliquots of the homogenate were used to measure the TG content and to prepare an acetone/ether powder to measure the LPL activity. The TG content was higher, but not significantly, in the sarcoma group than in the control group. The LPL activity of the sarcoma group was significantly higher than that of the control group. The TG content of the sarcoma group correlated positively with the LPL activity. [35S]Methionine incorporation investigation showed that the rate of LPL synthesis was significantly higher in the sarcoma group than in the control group. These results indicated that LPL was up-regulated at the transcriptional/translational level, thus resulting in an increased TG deposition in the adipose tissue of patients with muculoskeletal sarcoma. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved