RT Journal Article
SR Electronic
T1 Aberrant Gene Promoter Methylation in Sputum from Individuals Exposed to Smoky Coal Emissions
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2061
OP 2066
VO 28
IS 4B
A1 YANG LIU
A1 QING LAN
A1 MIN SHEN
A1 JIDE JIN
A1 JUDY MUMFORD
A1 DIANXU REN
A1 PHOUTHONE KEOHAVONG
YR 2008
UL http://ar.iiarjournals.org/content/28/4B/2061.abstract
AB Background: Recent studies suggested the potential for aberrant gene promoter methylation in sputum as a predictive marker for lung cancer. Here, the promoter methylation of p16, MGMT, RASSF1A and DAPK genes was investigated in sputum of individuals exposed to smoky coal emissions in Xuan Wei, China, where the lung cancer rate is more than 6 times the Chinese national average. Materials and Methods: Sputum DNA of 107 noncancer individuals and 58 lung cancer patients was screened for promoter methylation using methylation-specific PCR. Results: Promoter methylation of the p16 gene was detected in about half [51.4% (55/107)] of sputum DNA from noncancer individuals, a frequency higher than that observed for the RASSF1A (29.9%), MGMT (17.8%) and DAPK (15.9%) genes. Furthermore, the p16 gene was affected by promoter methylation at a frequency even higher among the lung cancer group, compared with the noncancer group [70.7% (41/58) versus 51.7% (55/107), p=0.017]. Conclusion: Individuals exposed to smoky coal emissions in this region harbored frequent promoter methylation of these genes in their sputum and some of such alterations may be involved in lung tumor development. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved