RT Journal Article SR Electronic T1 SPMSP: A Solid-phase PCR with Colorimetric Read-out for Quantitative Methylation Analysis JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 2055 OP 2060 VO 28 IS 4B A1 SONJA SIEVERS A1 CARLA FRITZSCH A1 CORNELIUS KUHNEN A1 OLIVER MÜLLER YR 2008 UL http://ar.iiarjournals.org/content/28/4B/2055.abstract AB Background: As DNA methylation has been determined as an important diagnostic biomarker in cancer management, methods for the detection of quantitative DNA methylation which are high-throughput and low-cost are needed. Materials and Methods: In this work, we introduce the solid-phase methylation-specific PCR (SPMSP) as a method for quantitative methylation analysis. SPMSP combines methylation-specific DNA amplification on a solid phase with subsequent colorimetric detection in an ELISA-based format. Results: In contrast to existing methods for quantitative methylation analysis, SPMSP can be carried out with standard laboratory equipment, i.e. a thermocycler and an ELISA reader. With this method, DNA methylation in the promoter of the APC tumour suppressor gene was quantified in a set of colorectal carcinoma samples. Conclusion: As SPMSP can be modified to the analysis of other promoter sequences and is also adaptable to a high-throughput system, SPMSP offers a platform for methylation profiling with widespread applications in cancer research and management. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved