RT Journal Article
SR Electronic
T1 Down-regulated Expression of RhoA in Human Conventional Renal Cell Carcinoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2039
OP 2043
VO 28
IS 4B
A1 YEONG-SHIAU PU
A1 CHUANG-WEI WANG
A1 GUANG-YAW LIU
A1 YI-ZIH KUO
A1 CHAO-YUAN HUANG
A1 WANG-YI KANG
A1 CHIA-TUNG SHUN
A1 CHIA-CHI LIN
A1 WEN-JENG WU
A1 TZYH-CHYUAN HOUR
YR 2008
UL http://ar.iiarjournals.org/content/28/4B/2039.abstract
AB Background: Renal cell carcinoma (RCC) is the most common cancer in adult kidney. Delayed diagnosis may result in progression and metastasis. Markers for early detection of RCC are lacking. The small GTPase RhoA has been implicated in the regulation of cell morphology, motility and transformation, but the role of RhoA in tumorigenesis of RCC remains unclear. In this study, the significance of RhoA expression in human RCC was characterized. Materials and Methods: RhoA mRNA and protein expressions were examined by real-time polymerase chain reaction (PCR) and immunohistochemistry respectively in 46 pairs of tissues. Results: Real-time PCR revealed that RhoA mRNA expression was significantly lower in cancerous as compared to normal tissues (p=0.036). RhoA protein expression was significantly higher in normal than in cancerous tissues (p=0.032). However, RhoA expression did not correlate with age, differentiation grade or TNM stage (all p>0.05). Conclusion: RhoA down-regulation may be involved in human renal tumorigenesis. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved