RT Journal Article
SR Electronic
T1 Impact of Antigen-unloaded Immature Dendritic Cells on Antileukemic T-Cell Cytotoxicity
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2831
OP 2835
VO 28
IS 5A
A1 ULF HARNACK
A1 GABRIELE PECHER
YR 2008
UL http://ar.iiarjournals.org/content/28/5A/2831.abstract
AB Background: Immature dendritic cells (iDC) loaded with antigens are able to induce tolerance in antigen-specific T-cells. The potential of antigen-unloaded iDC to regulate the antileukemic cytotoxicity of autologous T-cells was determined. Materials and Methods: iDC generated with 50 U/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) and very immature DC (viDC) generated with 10 U/ml GM-CSF from the bone marrow of Balb/c mice were used for T-cell co-culture. Results: The measurement of cellular cytotoxicity against the syngeneic murine B-cell leukemia line A20 revealed that T-cells without co-culture or after co-culture with iDC exerted a similar cytotoxicity, whereas T-cells co-incubated with viDC showed a significantly diminished lysis of A20 cells (p<0.05). Conclusion: Antigen-unloaded iDC in contrast to antigen-loaded iDC may not affect antileukemic T-cell cytotoxicity, whereas antigen-unloaded DC cultures generated with a low dose of GM-CSF are able to impair the T-cell-mediated cytolysis of leukemic cells.