PT  - JOURNAL ARTICLE
AU  - LIN, YUH-TZY
AU  - YANG, JAI-SING
AU  - LIN, SHUW-YUAN
AU  - TAN, TZU-WEI
AU  - HO, CHIN-CHIN
AU  - HSIA, TE-CHUN
AU  - CHIU, TSAN-HUNG
AU  - YU, CHUN-SHU
AU  - LU, HSU-FENG
AU  - WENG, YUEH-SHAN
AU  - CHUNG, JING-GUNG
TI  - Diallyl Disulfide (DADS) Induces Apoptosis in Human Cervical Cancer Ca Ski Cells <em>via</em> Reactive Oxygen Species and Ca<sup>2+</sup>-dependent Mitochondria-dependent Pathway
DP  - 2008 Sep 01
TA  - Anticancer Research
PG  - 2791--2799
VI  - 28
IP  - 5A
4099  - http://ar.iiarjournals.org/content/28/5A/2791.short
4100  - http://ar.iiarjournals.org/content/28/5A/2791.full
SO  - Anticancer Res2008 Sep 01; 28
AB  - The mechanisms of apoptosis induced by diallyl disulfide (DADS) were explored in human cervical cancer Ca Ski cells. Flow cytometric analysis, DNA gel electrophoresis and DAPI staining demonstrated that DADS induced apoptosis in Ca Ski cells. DADS induced apoptosis through the production of reactive oxygen species and Ca2+, and induced abrogation of mitochondrial membrane potential (Δψm) and cleavage of Bid protein (t-Bid). DADS increased the levels of p53, p21 and Bax, but caused a decrease in the level of Bcl-2. DADS also promoted the activities of caspase-3 leading to DNA fragmentation, thus indicating that DADS-induced apoptosis is caspase-3 dependent. In addition, DADS induced an increase in the level of cytochrome c in the cytoplasm, which was released from mitochondria. BAPTA attenuated the Δψm abrogation and significantly diminished the occurrence of DADS-induced apoptosis in Ca Ski cells. In conclusion, DADS-induced apoptosis occurs via production of ROS and caspase-3 and a mitochondria-dependent pathway in Ca Ski cells.