RT Journal Article
SR Electronic
T1 Mepacrine Antagonises Tumour Cell Growth Induced by Natural Polyamines
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2765
OP 2768
VO 28
IS 5A
A1 ROSSI, TIZIANA
A1 COPPI, ANDREA
A1 BRUNI, ELISA
A1 RUBERTO, ANTONIO
A1 GIUDICE, STEFANIA
A1 BAGGIO, GIOSUÉ
YR 2008
UL http://ar.iiarjournals.org/content/28/5A/2765.abstract
AB Background: Mepacrine is an antiproliferative agent, characterised by an aliphatic chain similar to that of natural polyamines whose activation is closely associated with cell proliferation and may lead to malignant transformation and neurodegenerative diseases. This study aims to investigate a possible antagonism between mepacrine and polyamines in tumour proliferation. Materials and Methods: MCF-7 and Vero cells were cultured in Eagle's minimum essential medium and then subjected to graded concentrations of putrescine, spermine and spermidine alone and in combination with mepacrine. Methyl thiazole tetrazolium test and Western-blotting were performed. Results: Putrescine and spermidine at 0.5 mg/l significantly stimulated cell growth, whereas mepacrine treatment confirmed the enhanced p21 expression previously reported by a recent study and growth inhibition. When used in combination, mepacrine antagonized MCF-7 growth induced by polyamines. Conclusion: Our results suggest that mepacrine may represent a choice in the treatment of tumours induced by the modified concentration of polyamines.