%0 Journal Article %A BORBÁLA RÉTHY %A JUDIT HOHMANN %A RENÁTA MINORICS %A ANDRÁS VARGA %A IMRE OCSOVSZKI %A JOSEPH MOLNÁR %A KATA JUHÁSZ %A GEORGE FALKAY %A ISTVÁN ZUPKÓ %T Antitumour Properties of Acridone Alkaloids on a Murine Lymphoma Cell Line %D 2008 %J Anticancer Research %P 2737-2743 %V 28 %N 5A %X The aim of the present study was to investigate the anticancer properties of a set of furanoacridone alkaloids, arborinine and evoxanthine, including the inhibitory effect of P-glycoprotein (Pgp) and the apoptosis-inducing capacity. The tested alkaloids were evaluated for multidrug resistance (MDR)-reversing activity on human Pgp-transfected L5178 mouse lymphoma cells, using the rhodamine-123 (Rh-123) assay. The antiproliferative effects of natural compounds and their interactions with doxorubicin were determined in MTT (3-(4,5-dimethylthiazol-2-yl) - 2,5-diphenyltetrazolium bromide) assays. Apoptosis-inducing activity was additionally measured by means of dual annexin V and propidium iodide staining. RT-PCR was used to test the expression of Pgp mRNA after acridone treatment. All of the acridones investigated increased the accumulation of Rh-123. Gravacridonetriol and gravacridonediol monomethyl ether increased the antiproliferative effect of doxorubicin on resistant L5178 cells. Treatment with these agents resulted in a decrease in Pgp mRNA levels. Naturally occurring acridone alkaloids exhibit a beneficial combination of anticancer effects and, accordingly, the acridone skeleton can be considered useful in the design of novel antiproliferative agents. %U https://ar.iiarjournals.org/content/anticanres/28/5A/2737.full.pdf