RT Journal Article
SR Electronic
T1 Promotion of the Self-renewal Capacity of Human Acute Leukemia Cells by Wnt3A
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2701
OP 2704
VO 28
IS 5A
A1 KAWAGUCHI-IHARA, NORIKO
A1 MUROHASHI, IKUO
A1 NARA, NOBUO
A1 TOHDA, SHUJI
YR 2008
UL http://ar.iiarjournals.org/content/28/5A/2701.abstract
AB Background: Wnt/β-catenin signaling is involved in the growth of various types of cancer cells. Wnt3A has been reported to promote the self-renewal of hematopoietic stem cells. Materials and Methods: The effects of recombinant Wnt3A protein on the in vitro growth of four acute myeloid leukemia (AML) and four acute T-lymphoblastic leukemia (T-ALL) cell lines was examined. Results: Wnt3A stimulation either had no effect on, or slightly suppressed, the short-term growth of these cell lines. In three cell lines, Wnt3A promoted clonogenic cell recovery after suspension culture, suggesting the promotion of the self-renewal capacity of leukemic stem or progenitor cells. Immunoblot analysis showed that Wnt3A stimulation reduced phosphorylated β-catenin and increased β-catenin in these cells, indicating that Wnt3A stimulation activated Wnt/β-catenin signaling. Conclusion: Wnt3A stimulation did not promote the growth of whole cell populations, but did promote the self-renewal of leukemic stem/progenitor cells in some AML and T-ALL cell lines.