RT Journal Article
SR Electronic
T1 Targeting of CDC20 <em>via</em> Small Interfering RNA Causes Enhancement of the Cytotoxicity of Chemoradiation
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1559
OP 1563
VO 28
IS 3A
A1 TANIGUCHI, KOICHI
A1 MOMIYAMA, NOBUYOSHI
A1 UEDA, MICHIO
A1 MATSUYAMA, RYUSEI
A1 MORI, RYUTARO
A1 FUJII, YOSHIRO
A1 ICHIKAWA, YASUSHI
A1 ENDO, ITARU
A1 TOGO, SHINJI
A1 SHIMADA, HIROSHI
YR 2008
UL http://ar.iiarjournals.org/content/28/3A/1559.abstract
AB Background: Cell division cycle 20 homologue (CDC20), which encodes a protein that promotes chromosomal separation, is highly expressed in several carcinomas, including pancreatic cancer. Materials and Methods: To ascertain whether this gene could be a potential therapeutic target, the RNA interference technique was applied using small interfering RNA (siRNA) to knockdown CDC20 expression. Results: The CDC20 siRNA showed more than 90% inhibition of CDC20 expression at both the transcriptional and translational levels and the specific knockdown of CDC20 expression inhibited the cell growth of human pancreatic carcinoma cells in vitro. Suppression of CDC20 induced accumulation of the cells in the G2/M-phase of the cell cycle. In addition, the knockdown of CDC20 caused enhancement of the cytotoxicity of paclitaxel and increased the effect of γ-irradiation against pancreatic carcinoma cells. Conclusion: CDC20 is a promising target for gene specific therapy in human pancreatic cancer. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved