RT Journal Article
SR Electronic
T1 Combined Applications of Repurposed Drugs and Their Detrimental Effects on Glioblastoma Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 207
OP 214
DO 10.21873/anticanres.13099
VO 39
IS 1
A1 ALEKSANDRA SACHKOVA
A1 SWETLANA SPERLING
A1 DOROTHEE MIELKE
A1 BAWARJAN SCHATLO
A1 VEIT ROHDE
A1 MILENA NINKOVIC
YR 2019
UL http://ar.iiarjournals.org/content/39/1/207.abstract
AB Background/Aim: Glioblastoma multiforme (GBM) is a malignant primary brain tumor with high rates of recurrence. This study aimed to investigate the effect of repurposed drug combinations on GBM. Materials and Methods: Viability of U87 MG and 11ST patient-derived GMB cell lines, after valproic acid, tranylcypromine or riluzole alone, in different combinations, as well as combined with standard temozolomide chemotherapy was examined using the MTT assay. Proliferation, mRNA level of tissue factor pathway inhibitor 2 (TFPI2), and cell invasion were evaluated using anti-Ki-67 antibody staining, reverse transcriptase-polymerase chain reaction and xCELLigence system. Results: The strongest effect on cell viability was achieved by the combination of riluzole with valproic acid (U87MG: 27.2%, 11ST: 25.99%). Tranylcypromine significantly enhanced the effect of temozolomide when used in combination, as did valproic acid. The normally high proliferation of GBM significantly declined under treatment with valproic acid with tranylcypromine (p=0.01). Finally, we observed reduction of invasion comparing single tranylcypromine to its combination with valproic acid or riluzole. Conclusion: These results support the idea that combinations of drugs could increase the treatment efficiency of GBM.