RT Journal Article SR Electronic T1 Prognostic Significance of the Local Expression of Interleukin-12 in Patients with Advanced Gastric Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 1277 OP 1283 VO 28 IS 2B A1 NOBUO NAGASHIMA A1 YOSHIFUMI NAKAYAMA A1 YUZURU INOUE A1 JUN NAGATA A1 KENTARO MATSUMOTO A1 NORITAKA MINAGAWA A1 TAKEFUMI KATSUKI A1 KAZUNORI SHIBAO A1 KEIJI HIRATA A1 TATSUHIKO SAKO A1 TETSUO HAMADA A1 NAOKI NAGATA YR 2008 UL http://ar.iiarjournals.org/content/28/2B/1277.abstract AB Background: Interleukin (IL)-12 is a heterodimeric cytokine that exhibits potent antitumor and antimetastatic activities. Very few studies have so far investigated the local expression of IL-12 in tumor specimens of gastric cancer. The purpose of this study was to investigate the immunohistochemical expression of IL-12 in patients with gastric cancer. Patients and Methods: IL-12 was immunohistochemically stained using monoclonal anti-human IL-12 antibody (1-1A4) in surgical specimens of 117 gastric cancer patients. The IL-12-positive cell density was calculated. The relationships among the IL-12-positive cell density, clinicopathological factors and 5-year survival rate were evaluated. Results: Among the patients (n=117), the 5-year survival rate after surgery was not statistically different between the patients with high and low IL-12 positive cell-density. However, in the patients with advanced gastric cancer (n=85), those with a high IL-12-positive cell density showed a significantly better prognosis in comparison with those with a low IL-12-positive cell density (p=0.0104). A multivariate analysis indicated that the IL-12-positive cell density and TNM stage are significant prognostic factors. Conclusion: IL-12-positive cell density may be a significant independent prognostic factor in surgical specimens of advanced gastric cancer. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved