TY - JOUR T1 - Comparison of Apolipoprotein D Determination Methods in Breast Cancer JF - Anticancer Research JO - Anticancer Res SP - 1151 LP - 1160 VL - 28 IS - 2B AU - HÅVARD SØILAND AU - IVAR SKALAND AU - EMIEL A.M. JANSSEN AU - EINAR GUDLAUGSSON AU - HARTWIG KÖRNER AU - JAN ERIK VARHAUG AU - JON ARNE SØREIDE AU - JAN P.A. BAAK Y1 - 2008/03/01 UR - http://ar.iiarjournals.org/content/28/2B/1151.abstract N2 - Background: Apolipoprotein D (ApoD) is a promising prognostic and predictive factor in breast cancer, but the analysis methods and results vary. Patients and Methods: Determination of ApoD content by immunoelectrophoresis in tumour cytosol (EPC), immunohistochemistry (IHC) in whole sections (WS) and tissue micro arrays (TMA) were compared in 283 breast carcinomas. Results: With EPC, 45% and with IHC, 71% of the tumours were ApoD-positive. Correlation between the degrees of ApoD positivity by ECP and IHC was poor (R2=0.04), caused by higher sensitivity of the IHC (resulting in many ECP negative carcinomas being IHC positive) and ApoD positivity of normal tissues and cysts (resulting in ApoD positivity by ECP in up to 33% of the IHC negative cases). Discrepancies between WS and TMA were considerable due to tumour heterogeneity. Conclusion: In breast cancer, IHC ApoD determination is superior to EPC analysis, but intratumor heterogeneity must be carefully considered when using TMA technology. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -