RT Journal Article SR Electronic T1 Comparison of Apolipoprotein D Determination Methods in Breast Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 1151 OP 1160 VO 28 IS 2B A1 HÅVARD SØILAND A1 IVAR SKALAND A1 EMIEL A.M. JANSSEN A1 EINAR GUDLAUGSSON A1 HARTWIG KÖRNER A1 JAN ERIK VARHAUG A1 JON ARNE SØREIDE A1 JAN P.A. BAAK YR 2008 UL http://ar.iiarjournals.org/content/28/2B/1151.abstract AB Background: Apolipoprotein D (ApoD) is a promising prognostic and predictive factor in breast cancer, but the analysis methods and results vary. Patients and Methods: Determination of ApoD content by immunoelectrophoresis in tumour cytosol (EPC), immunohistochemistry (IHC) in whole sections (WS) and tissue micro arrays (TMA) were compared in 283 breast carcinomas. Results: With EPC, 45% and with IHC, 71% of the tumours were ApoD-positive. Correlation between the degrees of ApoD positivity by ECP and IHC was poor (R2=0.04), caused by higher sensitivity of the IHC (resulting in many ECP negative carcinomas being IHC positive) and ApoD positivity of normal tissues and cysts (resulting in ApoD positivity by ECP in up to 33% of the IHC negative cases). Discrepancies between WS and TMA were considerable due to tumour heterogeneity. Conclusion: In breast cancer, IHC ApoD determination is superior to EPC analysis, but intratumor heterogeneity must be carefully considered when using TMA technology. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved