PT  - JOURNAL ARTICLE
AU  - LUZ SIEGEL
AU  - ANNE MITERNIQUE-GROSSE
AU  - CHRISTIAN GRIFFON
AU  - CLAUDINE KLEIN-SOYER
AU  - ANNELISE LOBSTEIN
AU  - FRANCIS RAUL
AU  - DOMINIQUE STEPHAN
TI  - Antiangiogenic Properties of Lupulone, a Bitter Acid of Hop Cones
DP  - 2008 Jan 01
TA  - Anticancer Research
PG  - 289--294
VI  - 28
IP  - 1A
4099  - http://ar.iiarjournals.org/content/28/1A/289.short
4100  - http://ar.iiarjournals.org/content/28/1A/289.full
SO  - Anticancer Res2008 Jan 01; 28
AB  - Background: Angiogenesis is the result of intricate steps regulated by the balance between agonistic and antagonistic effectors. Disturbance of this balance leads to an ‘angiogenic’ switch critical for tumor development. Materials and Methods: Using human umbilical vein endothelial cells (HUVEC) the effects of lupulone were analyzed on proliferation induced by angiogenic growth factors, transmembrane cell migration toward fibronectin and formation of a network of tubular-like structures on Matrigel. Results: Lupulone (2.5-50 μg/ml) induced a concentration-dependent inhibition of HUVEC proliferation and chemotaxis. Lupulone caused a significant reduction of closed capillary-like structures in Matrigel indicating a strong inhibitory effect on neovascularisation. In mice receiving lupulone (20 mg/kg/day) in drinking water for 21 days, new vessel formation was reduced by 50% in matrigel plugs implanted under the skin when compared with controls. Conclusion: The present data demonstrate that lupulone is able to inhibit angiogenesis in vitro and in vivo. Lupulone emerges as a potential chemopreventive agent when considering its strong antiangiogenic properties.