TY - JOUR T1 - Tumor-specific Cytotoxicity and Type of Cell Death Induced by β-Cyclodextrin Benzaldehyde Inclusion Compound JF - Anticancer Research JO - Anticancer Res SP - 229 LP - 236 VL - 28 IS - 1A AU - YU LIU AU - HIROSHI SAKAGAMI AU - KEN HASHIMOTO AU - HIROTAKA KIKUCHI AU - OSAMU AMANO AU - MARIKO ISHIHARA AU - YUMIKO KANDA AU - SHIRO KUNII AU - MUTSUYUKI KOCHI AU - WEI ZHANG AU - GUANGYAN YU Y1 - 2008/01/01 UR - http://ar.iiarjournals.org/content/28/1A/229.abstract N2 - The cytotoxicity of, β-cyclodextrin benzaldehyde inclusion compound (CDBA) against human normal and cancer cell lines was investigated. CDBA showed slightly higher cytotoxicity against human tumor cell lines, as compared to normal cells, with a tumor-specificity index of 2.2. Human myelogenous leukemia cell lines (HL-60, ML-1, KG-1) were the most sensitive to CDBA, followed by human oral squamous cell carcinoma (HSC-2, HSC-3, HSC-4) and human glioblastoma (T98G, U87MG). Human normal cells (gingival fibroblasts, pulp cells, periodontal ligament fibroblasts) were the most resistant. CDBA induced internucleosomal DNA fragmentation in HL-60 cells and caspase-3, -8, -9 activation, but to a much lesser extent than that attained by UV irradiation or actinomycin D. On the other hand, CDBA did not induce DNA fragmentation, nor caspase activation in HSC-2, HSC-4 or T98G cells. Electron microscopy demonstrated that CDBA induced the destruction of mitochondrial structure and digestion of broken organelles by secondary lysosomes in all of these cells. CDBA also increased the number of acidic organelles as judged by acridine orange staining. The present study suggests that CDBA induces autophagic cell death in cancer cell lines. ER -