RT Journal Article
SR Electronic
T1 PAI-1, t-PA and Circulating hTERT DNA as Related to Virus Infection in Liver Carcinogenesis
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 223
OP 228
VO 28
IS 1A
A1 DIVELLA, ROSA
A1 LACALAMITA, ROSANNA
A1 TOMMASI, STEFANIA
A1 COVIELLO, MARIA
A1 DANIELE, ANTONELLA
A1 GARRISI, VITO MICHELE
A1 ABBATE, INES
A1 SIMONE, GIOVANNI
A1 GADALETA, COSIMO
A1 PARADISO, ANGELO
A1 QUARANTA, MICHELE
YR 2008
UL http://ar.iiarjournals.org/content/28/1A/223.abstract
AB Background: Liver carcinogenesis seems to be heavely influenced by hepatitis B and C viral (HBV, HCV) infection. The aim of our study was to improve the detection of hepatocellular carcinoma (HCC) by measuring alfa-fetoprotein (AFP) in addition to other molecular markers by estimating the plasma levels of human catalytic fraction of reverse telomerase (hTERT) DNA, plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (t-PA) in 75 patients with liver desease. Patients and Methods: A control group was enrolled (N=30). PAI-1 and t-PA levels were detected with enzyme-linked immunoassorbent assay (ELISA), DNA hTERT was performed with real time polymerase chain reaction (RT-PCR). Results: PAI-1, t-PA and hTERT DNA levels were much higher than in controls. PAI-1 and t-PA levels were higher in the presence of both viruses compared to their absence, p<0.001. Moreover, hTERT was significantly higher in the presence of both viruses, p<0.05 and in the presence of HCV alone, p<0.05. No decrease or increase of AFP was noted in these patients. Conclusion: Our data suggest the reliability of PAI-1, t-PA and hTERT in detecting HCC, in particular when the carcinogenesis is affected by virus infection.