RT Journal Article SR Electronic T1 Frequent Splicing Aberration of the Base Excision Repair Gene hMYH in Human Gastric Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 215 OP 221 VO 28 IS 1A A1 KOBAYASHI, KATSUTOSHI A1 SHIDA, ATSUO A1 YAMADA, HISASHI A1 ISHIBASHI, YOSHIO A1 NAKAYAMA, RITSUKO A1 TORIUMI, YASUO A1 MITSUMORI, NORIO A1 KASHIWAGI, HIDEYUKI A1 YANAGA, KATSUHIKO YR 2008 UL http://ar.iiarjournals.org/content/28/1A/215.abstract AB Background: Missense mutation of hMYH, which prevents transversion mutations induced by oxidative DNA damage, is reportedly associated with the development of gastric and colon cancer. We investigated whether deficiency or mutation of hMYH is associated with gastric carcinogenesis. Patients and Methods: Thirty patients with gastric carcinoma, three gastric cancer cell lines and lymphocytes from three healthy volunteers were investigated. Reverse transcription-polymerase chain reaction (RT-PCR) was performed for hMYH, and the full-length sequence of hMYH mRNA was analysed. Results: A silent mutation at codon 473 was seen in two tumours. Single nucleotide polymorphism at codon 345 was observed in 14 patients. These two base substitutions had no pathogenic effect. Seven splice variants were observed and two aberrant transcripts were detected more frequently in cancer specimens (67%) than in normal mucosa (10%). Conclusion: The high frequency of splicing aberration in cancer tissues suggests that aberrant transcripts may be involved in gastric carcinogenesis and cancer development.