TY - JOUR T1 - Frequent Splicing Aberration of the Base Excision Repair Gene <em>hMYH</em> in Human Gastric Cancer JF - Anticancer Research JO - Anticancer Res SP - 215 LP - 221 VL - 28 IS - 1A AU - KATSUTOSHI KOBAYASHI AU - ATSUO SHIDA AU - HISASHI YAMADA AU - YOSHIO ISHIBASHI AU - RITSUKO NAKAYAMA AU - YASUO TORIUMI AU - NORIO MITSUMORI AU - HIDEYUKI KASHIWAGI AU - KATSUHIKO YANAGA Y1 - 2008/01/01 UR - http://ar.iiarjournals.org/content/28/1A/215.abstract N2 - Background: Missense mutation of hMYH, which prevents transversion mutations induced by oxidative DNA damage, is reportedly associated with the development of gastric and colon cancer. We investigated whether deficiency or mutation of hMYH is associated with gastric carcinogenesis. Patients and Methods: Thirty patients with gastric carcinoma, three gastric cancer cell lines and lymphocytes from three healthy volunteers were investigated. Reverse transcription-polymerase chain reaction (RT-PCR) was performed for hMYH, and the full-length sequence of hMYH mRNA was analysed. Results: A silent mutation at codon 473 was seen in two tumours. Single nucleotide polymorphism at codon 345 was observed in 14 patients. These two base substitutions had no pathogenic effect. Seven splice variants were observed and two aberrant transcripts were detected more frequently in cancer specimens (67%) than in normal mucosa (10%). Conclusion: The high frequency of splicing aberration in cancer tissues suggests that aberrant transcripts may be involved in gastric carcinogenesis and cancer development. ER -