RT Journal Article
SR Electronic
T1 Overexpression of the Mitotic Spindle Assembly Checkpoint Genes <em>hBUB1</em>, <em>hBUBR1</em> and <em>hMAD2</em> in Thyroid Carcinomas with Aggressive Nature
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 139
OP 144
VO 28
IS 1A
A1 WADA, NOBUYUKI
A1 YOSHIDA, AKIRA
A1 MIYAGI, YOHEI
A1 YAMAMOTO, TOSHIHARU
A1 NAKAYAMA, HIROTAKA
A1 SUGANUMA, NOBUYASU
A1 MATSUZU, KENICHI
A1 MASUDO, KATSUHIKO
A1 HIRAKAWA, SHOHEI
A1 RINO, YASUSHI
A1 MASUDA, MUNETAKA
A1 IMADA, TOSHIO
YR 2008
UL http://ar.iiarjournals.org/content/28/1A/139.abstract
AB Background: The mitotic spindle assembly checkpoint (MSAC) genes are responsible for preventing chromosome missegregation. MSAC gene expressions have been reported to be associated with tumor cell proliferation or unfavorable cancer behavior. The present study was conducted to preliminary investigate the MSAC gene expressions in thyroid neoplasms. Materials and Methods: Expression levels of MSAC genes (hBUB1, hBUBR1, hBUB3 and hMAD2) were evaluated in 9 follicular thyroid adenomas (FAs), 9 follicular thyroid carcinomas (FTCs), 21 papillary thyroid carcinomas (PTCs), 5 anaplastic (undifferentiated) thyroid carcinomas (ATCs) and 3 adjacent normal thyroid tissues (NTs) by real-time quantitative RT-PCR. These gene expressions were compared between undifferentiated thyroid carcinomas (ATCs) and differentiated thyroid carcinomas (DTCs) and between advanced DTCs and non-advanced DTCs. DTCs included PTCs and FTCs. Advanced DTCs were defined as carcinoma with aggressive nature such as extrathyroid extension, distant metastasis, recurrence or death from the disease. Results: MSAC gene expressions varied in different thyroid tumors and fell in the order of ATC, DTC (PTC and FTC), FA and NT. Carcinomas had higher expression compared to adenoma or normal tissue. hBUB1, hBUBR1 and hMAD2 expressions in ATCs were significantly higher than those in DTCs (p&lt;0.005). hBUBR1 and hMAD2 expressions in advanced DTCs were significantly higher than those in non-advanced DTCs (p&lt;0.05). Conclusion: The MSAC genes were overexpressed in thyroid carcinomas with aggressive nature. Further studies are required to clarify the relationship between the MSAC gene expressions and thyroid cancer behavior.