PT  - JOURNAL ARTICLE
AU  - HIROYUKI TAMIYA
AU  - AKIHISA MITANI
AU  - AKIRA SAITO
AU  - TARO ISHIMORI
AU  - MINAKO SAITO
AU  - HIDEAKI ISAGO
AU  - TAISUKE JO
AU  - YASUHIRO YAMAUCHI
AU  - GOH TANAKA
AU  - TAKAHIDE NAGASE
TI  - Exosomal MicroRNA Expression Profiling in Patients with Lung Adenocarcinoma-associated Malignant Pleural Effusion
AID  - 10.21873/anticanres.13039
DP  - 2018 Dec 01
TA  - Anticancer Research
PG  - 6707--6714
VI  - 38
IP  - 12
4099  - http://ar.iiarjournals.org/content/38/12/6707.short
4100  - http://ar.iiarjournals.org/content/38/12/6707.full
SO  - Anticancer Res2018 Dec 01; 38
AB  - Background/Aim: Cytological analysis for diagnosing malignant pleural effusion associated with lung adenocarcinoma (Ad-MPE) shows limited sensitivity and novel diagnostic biomarkers are needed. The aim of this study was to evaluate the profile of four microRNAs (miRNAs) in exosomes in Ad-MPE and benign (non-neoplastic) pleural effusion (BPE). Materials and Methods: A total of 56 pleural effusion samples from patients with lung adenocarcinoma and benign diseases were collected. Exosomal miRNA expressions were evaluated using real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: The expression levels of miR-182 and miR-210 were significantly higher in the Ad-MPE than in the BPE samples. The receiver operating characteristics curve analyses of miR-182 and miR-210 for diagnosis of Ad-MPE yielded areas under the receiver operating characteristic curves of 0.87 and 0.81, respectively. Conclusion: These miRNA signatures may have a diagnostic potential for differentiating Ad-MPE from BPE.