RT Journal Article SR Electronic T1 Evidence for a Tumour Suppressive Function of IGF1-binding Proteins in Human Breast Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 3513 OP 3518 VO 27 IS 5B A1 A. SUBRAMANIAN A1 A.K. SHARMA A1 D. BANERJEE A1 W.G. JIANG A1 K. MOKBEL YR 2007 UL http://ar.iiarjournals.org/content/27/5B/3513.abstract AB The role of the insulin like growth factor (IGF) system in various human malignancies has been well established. The aim of this study was to determine the levels of mRNA expression of insulin-like growth factor-binding protein (IGFBP)-1, -3 and -7 genes in benign and malignant breast tissue and explore their relationship with various prognostic parameters. Materials and Methods: Breast cancer tissue (n=127) and normal background tissue (n-33) were prospectively collected and analysed for levels of IGFBP-1, -3 and -7 mRNA using real-time Q-PCR. mRNA levels were then analysed against tumour grade, nodal status, Nottingham prognostic index (NPI)/TNM stage and tumour type. Results: For IGFBP-1 and -3, mRNA expression was higher in normal tissue. This was significant for IGFBP-1 when comparing NPI 3 with NPI 1 (p=0.050) and the normal group (p=0.040). With respect to TNM analysis, there was less IGFBP-1 mRNA when comparing TNM 3 with normal (p=0.017), TNM 1 (p=0.047) and TNM 2 (p=0.019) tumours. This was also found when comparing TNM 4 samples with normal tissue (p=0.017), TNM 1(p=0.046) and TNM 2 (p=0.019). For IGFBP-3 mRNA, there was less mRNA when comparing TNM3 with TNM 1 (p=0.017) and TNM 2 (p=0.050), and also less mRNA expression when comparing TNM 4 with TNM 1 (p=0.030). For IGFBP-7 mRNA, both TNM 1 (p=0.0077) and TNM 2 (p=0.015) had significantly more expression than TNM 3 samples. Conclusion: This study supports the role of IGFBP-1, -3 and -7 as potential tumour suppressor genes in human breast cancer. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved