PT  - JOURNAL ARTICLE
AU  - SHIMIZU, KIHIRO
AU  - KUDO, AKO
AU  - IIDA, TOKO
AU  - SATO, KEISUKE
AU  - NAKATA, AKIFUMI
AU  - KOMATU, KENICHI
AU  - TAKAI, KAZUKI
AU  - WAKAME, KOJI
TI  - Antioxidant Effects of <em>Bidens Pilosa</em> Extract Protect RAW 264.7 Cells from Cisplatin-induced Cytotoxicity
AID  - 10.21873/anticanres.17707
DP  - 2025 Aug 01
TA  - Anticancer Research
PG  - 3459--3467
VI  - 45
IP  - 8
4099  - http://ar.iiarjournals.org/content/45/8/3459.short
4100  - http://ar.iiarjournals.org/content/45/8/3459.full
SO  - Anticancer Res2025 Aug 01; 45
AB  - Background/Aim: Bidens pilosa (BP) is a medicinal plant that exerts antioxidant and antiallergic effects and is used to treat various diseases. Cisplatin (CDDP) is used for a variety of malignancies, but its use is limited by its side effects. In this study, the effect of BP on reducing CDDP toxicity was examined using RAW264.7 cells, which are normal macrophages.Materials and Methods: RAW 264.7 cells were treated with BP and cytotoxicity and antioxidant capacities were measured. RAW 264.7 cells were treated with CDDP with and without BP. The expression of antioxidant-related genes was measured by RT-PCR, whereas apoptosis and reactive oxygen species (ROS) were assessed by flow cytometry.Results: BP inhibited CDDP-induced cytotoxicity in RAW 264.7 cells. Antioxidant gene expression was significantly increased in cells treated with BP and CDDP. BP reduced intracellular ROS as well as the percentage of apoptotic cells in RAW 264.7 cells treated with BP and CDDP.Conclusion: BP reduces CDDP-induced cytotoxicity; these effects are the result of the antioxidant activity of BP.