TY - JOUR T1 - Preliminary Observation on the Cytotoxic Activity of New Chlorophyllin Derivative RCD on Human Tumour Cell Lines <em>In Vitro</em> JF - Anticancer Research JO - Anticancer Res SP - 1807 LP - 1812 DO - 10.21873/anticanres.13287 VL - 39 IS - 4 AU - ARNALDO PINELLI AU - PAOLO FERRARIO AU - SILVIO TRIVULZIO Y1 - 2019/04/01 UR - http://ar.iiarjournals.org/content/39/4/1807.abstract N2 - Background: Chlorophyllin is used in traditional Chinese medicine because of its anticancer properties. This article describes the preparation and cytotoxic activity of a reduced chlorophyllin derivative (RCD) on tumour cell lines. Materials and Methods: RCD was prepared by reducing chlorophyllin with lithium aluminium hydride, and its solubility in the alkaline and organic phases are different from that of the parent compound chlorophyllin. Results: RCD also has different chromatographic behaviour from chlorophyllin on thin-layer chromatography and high-pressure liquid chromatography, and excitation and emission spectra. RCD has cytotoxic activity against ZR-75, MCF-7 and HT-29, human tumour cell lines. A clonogenic assay showed that the growth of tumour colonies on soft agar was reduced by RCD in a dose-dependent manner. Conclusion: RCD is a novel compound exhibiting anticancer activity. ER -