PT  - JOURNAL ARTICLE
AU  - ARNALDO PINELLI
AU  - PAOLO FERRARIO
AU  - SILVIO TRIVULZIO
TI  - Preliminary Observation on the Cytotoxic Activity of New Chlorophyllin Derivative RCD on Human Tumour Cell Lines <em>In Vitro</em>
AID  - 10.21873/anticanres.13287
DP  - 2019 Apr 01
TA  - Anticancer Research
PG  - 1807--1812
VI  - 39
IP  - 4
4099  - http://ar.iiarjournals.org/content/39/4/1807.short
4100  - http://ar.iiarjournals.org/content/39/4/1807.full
SO  - Anticancer Res2019 Apr 01; 39
AB  - Background: Chlorophyllin is used in traditional Chinese medicine because of its anticancer properties. This article describes the preparation and cytotoxic activity of a reduced chlorophyllin derivative (RCD) on tumour cell lines. Materials and Methods: RCD was prepared by reducing chlorophyllin with lithium aluminium hydride, and its solubility in the alkaline and organic phases are different from that of the parent compound chlorophyllin. Results: RCD also has different chromatographic behaviour from chlorophyllin on thin-layer chromatography and high-pressure liquid chromatography, and excitation and emission spectra. RCD has cytotoxic activity against ZR-75, MCF-7 and HT-29, human tumour cell lines. A clonogenic assay showed that the growth of tumour colonies on soft agar was reduced by RCD in a dose-dependent manner. Conclusion: RCD is a novel compound exhibiting anticancer activity.