@article {PINELLI1807, author = {ARNALDO PINELLI and PAOLO FERRARIO and SILVIO TRIVULZIO}, title = {Preliminary Observation on the Cytotoxic Activity of New Chlorophyllin Derivative RCD on Human Tumour Cell Lines In Vitro}, volume = {39}, number = {4}, pages = {1807--1812}, year = {2019}, doi = {10.21873/anticanres.13287}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Chlorophyllin is used in traditional Chinese medicine because of its anticancer properties. This article describes the preparation and cytotoxic activity of a reduced chlorophyllin derivative (RCD) on tumour cell lines. Materials and Methods: RCD was prepared by reducing chlorophyllin with lithium aluminium hydride, and its solubility in the alkaline and organic phases are different from that of the parent compound chlorophyllin. Results: RCD also has different chromatographic behaviour from chlorophyllin on thin-layer chromatography and high-pressure liquid chromatography, and excitation and emission spectra. RCD has cytotoxic activity against ZR-75, MCF-7 and HT-29, human tumour cell lines. A clonogenic assay showed that the growth of tumour colonies on soft agar was reduced by RCD in a dose-dependent manner. Conclusion: RCD is a novel compound exhibiting anticancer activity.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/39/4/1807}, eprint = {https://ar.iiarjournals.org/content/39/4/1807.full.pdf}, journal = {Anticancer Research} }