RT Journal Article
SR Electronic
T1 Altered Gene Expression by Cisplatin in a Human Squamous Cell Lung Carcinoma Cell Line
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3235
OP 3243
VO 27
IS 5A
A1 YATOMI, MARI
A1 TAKIGUCHI, YUICHI
A1 ASAKA-AMANO, YOSHIKO
A1 ARAI, MAKOTO
A1 TADA, YUJI
A1 KUROSU, KATSUSHI
A1 SAKAO, SEIICHIRO
A1 KASAHARA, YASUNORI
A1 TANABE, NOBUHIRO
A1 TATSUMI, KOICHIRO
A1 SEKI, NAOHIKO
A1 KURIYAMA, TAKAYUKI
YR 2007
UL http://ar.iiarjournals.org/content/27/5A/3235.abstract
AB Background: Substantial evidence has disclosed that some cytotoxic agents have complex activities in influencing signal transduction pathways in cells. Materials and Methods: cDNA microarray analysis was performed after exposing a human squamous cell carcinoma cell line, RERF-LC-AI, to low-dose cisplatin for 5 days. Up-regulated gene expressions were suppressed by small interfering RNA to investigate phenotypic alteration of the cells. Results: Among 30,000 genes screened, 42 genes showed increases or decreases in expression of more than 2-fold with cisplatin treatment. They included genes with functions involved in apoptosis, cell cycle regulation and DNA metabolism/repair. Suppression of the 5 most significantly altered genes by small interfering RNA resulted in partly reduced apoptosis without altering cytotoxicity of cisplatin. Conclusion: Besides direct cytotoxic effects on cells, cisplatin may have indirect effects involving drug resistance, and synergistic effects with other agents. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved