PT  - JOURNAL ARTICLE
AU  - YATOMI, MARI
AU  - TAKIGUCHI, YUICHI
AU  - ASAKA-AMANO, YOSHIKO
AU  - ARAI, MAKOTO
AU  - TADA, YUJI
AU  - KUROSU, KATSUSHI
AU  - SAKAO, SEIICHIRO
AU  - KASAHARA, YASUNORI
AU  - TANABE, NOBUHIRO
AU  - TATSUMI, KOICHIRO
AU  - SEKI, NAOHIKO
AU  - KURIYAMA, TAKAYUKI
TI  - Altered Gene Expression by Cisplatin in a Human Squamous Cell Lung Carcinoma Cell Line
DP  - 2007 Sep 01
TA  - Anticancer Research
PG  - 3235--3243
VI  - 27
IP  - 5A
4099  - http://ar.iiarjournals.org/content/27/5A/3235.short
4100  - http://ar.iiarjournals.org/content/27/5A/3235.full
SO  - Anticancer Res2007 Sep 01; 27
AB  - Background: Substantial evidence has disclosed that some cytotoxic agents have complex activities in influencing signal transduction pathways in cells. Materials and Methods: cDNA microarray analysis was performed after exposing a human squamous cell carcinoma cell line, RERF-LC-AI, to low-dose cisplatin for 5 days. Up-regulated gene expressions were suppressed by small interfering RNA to investigate phenotypic alteration of the cells. Results: Among 30,000 genes screened, 42 genes showed increases or decreases in expression of more than 2-fold with cisplatin treatment. They included genes with functions involved in apoptosis, cell cycle regulation and DNA metabolism/repair. Suppression of the 5 most significantly altered genes by small interfering RNA resulted in partly reduced apoptosis without altering cytotoxicity of cisplatin. Conclusion: Besides direct cytotoxic effects on cells, cisplatin may have indirect effects involving drug resistance, and synergistic effects with other agents. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved