RT Journal Article
SR Electronic
T1 Gene Expression Profiling Identifies New Biological Markers of Neoplastic Germ Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3091
OP 3100
VO 27
IS 5A
A1 BIERMANN, KATHARINA
A1 HEUKAMP, LUKAS CARL
A1 STEGER, KLAUS
A1 ZHOU, HUI
A1 FRANKE, FOLKER ERNST
A1 GUETGEMANN, INES
A1 SONNACK, VIOLETTA
A1 BREHM, RALPH
A1 BERG, JOHANNES
A1 BASTIAN, PATRICK JAN
A1 MÜLLER, STEFAN CAJETAN
A1 WANG-ECKERT, LIHUA
A1 SCHORLE, HUBERT
A1 BÜTTNER, REINHARD
YR 2007
UL http://ar.iiarjournals.org/content/27/5A/3091.abstract
AB Background: There is only limited knowledge about gene expression in human testicular germ cell tumors of adolescents and adults (TGCTs), and, in particular in its pre-invasive stage intratubular germ cell neoplasia unclassified (IGCNU). Materials and Methods: Global gene expression was studied in 10 invasive human testicular germ cell tumors (TGCTs), 7 intratubular germ cell neoplasia unclassified (IGCNU) and 3 normal testes. The pattern of expression of several genes was studied by immunohistochemistry on tissue microarrays containing 126 TGCTs, IGCNU, normal testes and in 5 fetal testes. Results: RAS-related genes (KRAS2, RALA, RAB39B) and various core markers of embryonic stem cells were overexpressed in IGCNU compared to normal testes. CD9, PODXL and centromere-specific histone-H3-like protein CENPA were specifically identified in IGCNU, seminomas, embryonal carcinomas and in fetal gonocytes. Embryonic stem cell regulator SOX2 and downstream targets of the Nodal pathway were up-regulated in embryonal carcinoma only but not in IGCNU/seminoma. Preliminary data revealed that the expression profile of IGCNU is dependent on the histology of the adjacent invasive tumor. Conclusion: Our study determined the genes involved in early pathogenetic events of neoplastic germ cell formation, provided new insights into genetic pathways driving the transition of embryonal carcinoma and seminoma from IGCNU and identified new biomarkers of neoplastic germ cells such as CD9, CENPA and PODXL. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved