RT Journal Article
SR Electronic
T1 New Methodological Approach to Induce a Differentiation Phenotype in Caco-2 Cells Prior to Post-confluence Stage
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3919
OP 3925
VO 27
IS 6B
A1 FERRARETTO, ANITA
A1 GRAVAGHI, CLAUDIA
A1 DONETTI, ELENA
A1 COSENTINO, STEFANIA
A1 DONIDA, BIANCA M.
A1 BEDONI, MARZIA
A1 LOMBARDI, GIOVANNI
A1 FIORILLI, AMELIA
A1 TETTAMANTI, GUIDO
YR 2007
UL http://ar.iiarjournals.org/content/27/6B/3919.abstract
AB Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved