<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">FERRARETTO, ANITA</style></author><author><style face="normal" font="default" size="100%">GRAVAGHI, CLAUDIA</style></author><author><style face="normal" font="default" size="100%">DONETTI, ELENA</style></author><author><style face="normal" font="default" size="100%">COSENTINO, STEFANIA</style></author><author><style face="normal" font="default" size="100%">DONIDA, BIANCA M.</style></author><author><style face="normal" font="default" size="100%">BEDONI, MARZIA</style></author><author><style face="normal" font="default" size="100%">LOMBARDI, GIOVANNI</style></author><author><style face="normal" font="default" size="100%">FIORILLI, AMELIA</style></author><author><style face="normal" font="default" size="100%">TETTAMANTI, GUIDO</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">New Methodological Approach to Induce a Differentiation Phenotype in Caco-2 Cells Prior to Post-confluence Stage</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007-11-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">3919-3925</style></pages><volume><style face="normal" font="default" size="100%">27</style></volume><issue><style face="normal" font="default" size="100%">6B</style></issue><abstract><style  face="normal" font="default" size="100%">Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved</style></abstract></record></records></xml>