PT  - JOURNAL ARTICLE
AU  - S. WÖHRER
AU  - W. RABITSCH
AU  - M. SHEHATA
AU  - R. KONDO
AU  - H. ESTERBAUER
AU  - B. STREUBEL
AU  - C. SILLABER
AU  - M. RADERER
AU  - U. JAEGER
AU  - C. ZIELINSKI
AU  - P. VALENT
TI  - Mesenchymal Stem Cells in Patients with Chronic Myelogenous Leukaemia or Bi-phenotypic Ph+ Acute Leukaemia are not Related to the Leukaemic Clone
DP  - 2007 Nov 01
TA  - Anticancer Research
PG  - 3837--3841
VI  - 27
IP  - 6B
4099  - http://ar.iiarjournals.org/content/27/6B/3837.short
4100  - http://ar.iiarjournals.org/content/27/6B/3837.full
SO  - Anticancer Res2007 Nov 01; 27
AB  - Background: Human mesenchymal stem cells (MSCs) are thought to be multipotent cells which primarily reside in the bone marrow. Besides their well-known ability to replicate as undifferentiated cells and to differentiate into diverse lineages of mesenchymal tissues, they were recently suggested to also give rise to haematopoietic and leukaemic/cancer stem cells. In this study, the relationship between MSCs and leukemic stem cells in patients with either chronic myelogenous leukaemia (CML) or the more primitive variant, Ph+ bi-phenotypic leukaemia was investigated. Patients and Methods: Cultured MSCs from 5 patients with CML and 3 patients with bi-phenotypic Ph+ leukaemia, all of them positive for BCR-ABL, were analysed with conventional cytogenetics, fluorescence in situ hybridisation (FISH) and polymerase chain reaction (PCR) for the presence of t(9;22) and BCR-ABL. MSCs were characterised phenotypically with surface markers (+CD73, +CD90, +CD105, -CD34, -CD45) and functionally through their potential to differentiate into both adipocytes and osteoblasts. Results: MSCs could be cultivated from seven patients. These cells were BCR-ABL negative when analysed with conventional cytogenetics and FISH. Further cytogenetic analysis revealed a normal set of chromosomes without any aberrations. Two patients were BCR-ABL-positive when analysed with PCR, probably as a result of MSC contamination with macrophages. Conclusion: MSCs in patients with CML or Ph+ bi-phenotypic leukaemia are not related to the malignant cell clone. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved