TY - JOUR T1 - Anti-patched-1 Antibodies Suppress Hedgehog Signaling Pathway and Pancreatic Cancer Proliferation JF - Anticancer Research JO - Anticancer Res SP - 3743 LP - 3747 VL - 27 IS - 6A AU - MASAFUMI NAKAMURA AU - MAKOTO KUBO AU - KOSUKE YANAI AU - YOSHIKO MIKAMI AU - MIO IKEBE AU - SHUNTARO NAGAI AU - KOJI YAMAGUCHI AU - MASAO TANAKA AU - MITSUO KATANO Y1 - 2007/11/01 UR - http://ar.iiarjournals.org/content/27/6A/3743.abstract N2 - Background: The hedgehog (Hh) signaling pathway is aberrantly activated in many human carcinomas including pancreatic cancer and regulates tumor cell growth. Over-production of sonic hedgehog (Shh), a ligand of the Hh signaling pathway, increases the Hh signaling activity through transmitting the signal to patched-1 (Ptch1), the receptor of the Hh signaling pathway. Materials and Methods: α-Ptch1 antibodies were raised against an oligo-peptide, designed according to the Ptch1 amino-acid sequence. The specificity of α-Ptch1 was examined by immunoblotting and immuno-fluorescence, and biological effects were detected by RT-PCR and cell proliferation assay using two pancreatic cancer cell lines, Panc1 and SUIT-2. Results: α-Ptch1 recognized a 160 kDa protein as shown by immunoblotting and cell surface staining of pancreatic cancer cells. Incubation with α-Ptch1 suppressed Hh signaling activity and proliferation of pancreatic cancer cells. Conclusion: These results provide a new strategy for controling Hh dependent development of pancreatic cancer and other Hh related carcinomas. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -