PT  - JOURNAL ARTICLE
AU  - INZKIRWELI, NATALJA
AU  - GÜCKEL, BRIGITTE
AU  - SOHN, CHRISTOF
AU  - WALLWIENER, DIETHELM
AU  - BASTERT, GUNTHER
AU  - LINDNER, MATTHIAS
TI  - Antigen Loading of Dendritic Cells with Apoptotic Tumor Cell-Preparations is Superior to that Using Necrotic Cells or Tumor Lysates
DP  - 2007 Jul 01
TA  - Anticancer Research
PG  - 2121--2129
VI  - 27
IP  - 4B
4099  - http://ar.iiarjournals.org/content/27/4B/2121.short
4100  - http://ar.iiarjournals.org/content/27/4B/2121.full
SO  - Anticancer Res2007 Jul 01; 27
AB  - Background: Loading of dendritic cells (DCs) with tumor cell (TC) preparations is an attractive method for vaccine preparation because the entire antigen repertoire of a tumor is processed and presented by the DCs, thus allowing the simultaneous stimulation of T-helper cells and cytotoxic T-lymphocytes. However, optimal loading conditions have still to be defined. Materials and Methods: DCs were pulsed either with tumor lysates, apoptotic or necrotic preparations of a breast cancer cell line and subsequently used to stimulate autologous T-lymphocytes. Antigen loading was quantified using immunofluorescent-based methods. Results: Four hours co-incubation of apoptotic TCs or tumor lysates with DCs undergoing maturation resulted in effective DC-loading. However, the DCs pulsed with apoptotic TCs were best in stimulating interferon-Á (INF-Á) secretion as the effector function of autologous T-cells. Conclusion: Tumor lysates are in common use for DC-based vaccine manufacturing. However, our data indicate an advantage of apoptotic TC-preparations in regard to antigen loading effectiveness as well as the loaded DC's capacity to activate T-cells. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved