<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">CHEN, JIN-CHERNG</style></author><author><style face="normal" font="default" size="100%">CHEN, YUN</style></author><author><style face="normal" font="default" size="100%">SU, YEN-HAO</style></author><author><style face="normal" font="default" size="100%">TSENG, SHENG-HONG</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Celecoxib Increased Expression of 14-3-3Û and Induced Apoptosis of Glioma Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007-07-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">2547-2554</style></pages><volume><style face="normal" font="default" size="100%">27</style></volume><issue><style face="normal" font="default" size="100%">4B</style></issue><abstract><style  face="normal" font="default" size="100%">Background: Celecoxib, a cyclooxygenase-2 inhibitor, has been found to inhibit the proliferation of several kinds of cancer cells; however, the effects of celecoxib on glioma cells are not clear. Materials and Methods: A172 glioma cells were treated with various concentrations of celecoxib for 4, 24 or 48 h. Cytotoxic drug effects were studied by MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide)-based colorimetric assay, and celecoxib-induced apoptosis of glioma cells was investigated by FACScan. Western blot analysis was used to study celecoxib effects on the expression of mitogen-activated protein kinases (MAPKs), p53, p21, 14-3-4Û, Bcl-2 and Bax. Caspace-3 activity in glioma cells was analyzed by caspase activity assay. Results: Celecoxib exerted cytotoxic effects upon and induced apoptosis of the A172 glioma cells in a concentration and time-dependent manner (p&lt;0.05). Celecoxib had no effects on expression of MAPKs, Bax, or p21; however, it increased expression of p53 and 14-3-4Û, and reduced expression of Bcl-2. Celecoxib also increased the activity of caspace-3 in glioma cells. The apoptotic fraction of A172 cells induced by 24-h treatment with 100 μM celecoxib was reduced from 39% to 23% by pretreatment with caspace-3 inhibitor (DEVD-CHO) (p&lt;0.001). Conclusion: The results suggest that celecoxib induced cytotoxicity and apoptosis in this line of glioma cells and that such effects might be related to activation of p53 and 14-3-3Û, reduced Bcl-2 and Bcl-2/Bax ratio, and increased caspace-3 activity. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved</style></abstract></record></records></xml>