RT Journal Article
SR Electronic
T1 CD47 and Calreticulin Expression in Breast Cancer Subtypes and Anti-CD47 Inhibitory Effects in Macrophage-mediated Phagocytosis
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4929
OP 4940
DO 10.21873/anticanres.17318
VO 44
IS 11
A1 CHANTARAAMPORN, JUTHAMARD
A1 POTHIPAN, PHATTARIN
A1 SAKULTERDKIAT, TITIPATIMA
A1 KHIANKAEW, BURANA
A1 LUMKUL, LALITA
A1 MUTAPAT, PHOTSATHORN
A1 PHETCHAHWANG, PHICHAMON
A1 SVASTI, JISNUSON
A1 CHAMPATTANACHAI, VORARATT
YR 2024
UL http://ar.iiarjournals.org/content/44/11/4929.abstract
AB Background/Aim: Macrophage-mediated cancer immune evasion is modulated by the balance between “the cluster of differentiation 47 (CD47), an anti-phagocytic signal” and “calreticulin (CALR), a pro-phagocytic signal”. CD47 is highly expressed in various types of cancer. However, the expression profiles of CD47 and CALR in breast cancer, especially in different hormone receptor subtypes, and the effects of CD47 blockade in macrophage-mediated therapy are not well understood. Materials and Methods: The expression levels of CD47 and CALR were investigated in breast cancer and adjacent normal tissues using immunohistochemistry. To study the effects of CD47 blockade therapy, CD47 and CALR expression in breast cancer cell lines were determined. In vitro macrophage-mediated phagocytosis of breast cancer upon treatment with a monoclonal CD47 antibody (B6H12) were performed. Results: CD47 and CALR were overexpressed in breast cancer tissues and their up-regulation was associated with hormone receptor subtypes. Patients with Luminal A breast cancer had higher levels of CD47, while patients with triple-negative breast cancer (TNBC) had higher levels of CALR. The levels of CD47 and CALR were also elevated in breast cancer cell lines of Luminal A (MCF-7) and TNBC (MDA-MB-231) subtypes. Interestingly, the expression ratio of surface CD47/CALR was significantly higher in MCF-7. Moreover, in vitro phagocytosis assays revealed that blockage of CD47 enhanced macrophage-mediated activity in both cancer cells with dramatically higher degrees of phagocytosis in MCF-7. Conclusion: The expression profiles of CD47 and CALR in breast cancer subtypes and the benefit of CD47 blocking-based immunotherapy are herein provided.