RT Journal Article
SR Electronic
T1 Ethyl 2-[N-m-chlorobenzyl-(2′-methyl)]anilino-4-oxo-4,5-dihydrofuran-3-carboxylate (JOT01006) Induces Apoptosis in Human Cervical Cancer HeLa Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2505
OP 2514
VO 27
IS 4B
A1 HUANG, AN-CHENG
A1 LIN, TSUNG-PING
A1 WENG, YUEH-SHAN
A1 HO, YUNG-TSUAN
A1 LIN, HUI-JU
A1 HUANG, LI-JIAU
A1 KUO, SHENG-CHU
A1 CHUNG, JING-GUNG
YR 2007
UL http://ar.iiarjournals.org/content/27/4B/2505.abstract
AB Human cervical cancer is potentially lethal, and therefore the development of effective and tolerable therapeutic options is vital. In the present study, the in vitro effect of the synthetized compound JOT01006 (C21H20C1NO4) on human cervical epithelioid carcinoma cell line (HeLa) was examined. The results demonstrated that JOT01006 induced morphological changes and cytotoxicity (decreased the percentage of viable cells) in a dose-dependent manner. JOT01006 induced apoptosis which was analyzed by flow cytometric methods and confirmed by DAPI staining and DNA fragmentation analyzed by DNA gel electrophoresis. JOT01006 also induced reactive oxygen species (ROS) overproduction before causing endoplasmic reticulum (ER) stress which was also confirmed by the increased levels of Grp78 and Gadd153. Western blotting was selected to demonstrate that JOT01006 promoted p53, Bak, PARP, caspase-3 levels and decreased the levels of Bcl-2 and Bcl-xL. Our results also showed that JOT01006 also promoted caspase-12 production followed by apoptosis. The results also showed that JOT01006 inhibited the migration of HeLa cells potentially through inhibition of MMP-2 and -9. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved