TY - JOUR T1 - Serum Markers to Detect Metastatic Uveal Melanoma JF - Anticancer Research JO - Anticancer Res SP - 1897 LP - 1900 VL - 27 IS - 4A AU - VIVIAN BARAK AU - SHACHAR FRENKEL AU - INNA KALICKMAN AU - ANDREW J. MANIOTIS AU - ROBERT FOLBERG AU - JACOB PE'ER Y1 - 2007/07/01 UR - http://ar.iiarjournals.org/content/27/4A/1897.abstract N2 - Background: Osteopontin (OPN) is overexpressed in metastatic uveal melanoma (UM). S-100β and melanoma-inhibitory activity (MIA) serum levels are elevated in metastatic cutaneous melanoma. The ability of OPN, S-100β and MIA serum levels to be used as non-invasive markers for detecting metastatic UM was tested. Patients and Methods: OPN, S-100β and MIA levels were measured by ELISA assays in 18 patients with metastatic UM and in 38 patients who were disease-free (DF) for at least 10 years after treatment of the primary tumor. Paired serum samples from 8 patients before and after development of metastasis were analyzed. Forty-four healthy controls (C) were compared to the other two groups. Results: Serum OPN, MIA, and S-100β levels were significantly higher in patients with metastatic UM as compared to patients who were DF for at least 10 years after treatment (p=0.0001) or with age-matched controls. Serum OPN, MIA and S-100β levels were significantly higher (p<0.005) after metastasis formation than before the clinical detection of metastasis in the 8 patients. Receiver operator characteristic analysis was performed for metastatic patients vs. DF and vs. C, and the area under the curve was calculated for each marker and for the combination of the 3 markers, which was 91%. Conclusion: Elevated serum OPN, MIA and S-100β levels correlate with metastatic UM to the liver. When used in combination, these markers provide a highly sensitive and specific method to detect hepatic metastases and therefore provide for earlier therapeutic intervention that can prolong survival. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -