RT Journal Article SR Electronic T1 Transcriptional Induction of SERPINE1 and Fibrinolysis Inhibition as Predominant Effects of Glucocorticoids on the Cancer Coagulome JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 3269 OP 3276 DO 10.21873/anticanres.17145 VO 44 IS 8 A1 RACINE, FLORIANE A1 LOUANDRE, CHRISTOPHE A1 DEMAGNY, JULIEN A1 GODIN, CORINNE A1 SAIDAK, ZUZANA A1 GALMICHE, ANTOINE YR 2024 UL http://ar.iiarjournals.org/content/44/8/3269.abstract AB Background/Aim: How tumors regulate the genes of the coagulome is crucial for cancer-associated thrombosis and the occurrence of venous thromboembolic complications in patients with cancer. We have previously reported potent yet complex effects of glucocorticoids (GC) on the expression of three genes that play a key role in the regulation of thrombin/plasmin activation (F3, PLAU, and SERPINE1). This study aimed to extend the investigation of GC effects to the whole tumor coagulome and assess the resulting impact on the ability of cancer cells to activate thrombin and plasmin. Materials and Methods: Cancer RNA expression data were retrieved from various sources. Additionally, oral squamous cell carcinoma (OSCC) cells exposed to GC in vitro were analyzed using QPCR, enzymatic assays measuring thrombin and urokinase-type Plasminogen Activator (uPA) activity, and D-dimer concentrations. Results: Our findings highlight the potent and specific stimulatory effect of GC on SERPINE1 expression across different types of cancer. Consistently, GC were found to inhibit uPA proteolytic activity and reduce the concentrations of D-dimers in OSCC in vitro. Conclusion: Fibrinolysis inhibition is a key consequence of cancer cell exposure to GC, possibly leading to the stabilization of the fibrin clot in cancer.