RT Journal Article
SR Electronic
T1 Proteomic Analysis to Dissect Mitoxantrone Resistance-associated Proteins in a Squamous Lung Carcinoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1277
OP 1284
VO 27
IS 3A
A1 MURPHY, L.
A1 CLYNES, M.
A1 KEENAN, J.
YR 2007
UL http://ar.iiarjournals.org/content/27/3A/1277.abstract
AB Background: Mitoxantrone resistance has been related to the expression of a drug efflux pump breast cancer resistance pump (BCRP) but little is known of the intracellular protein changes. In this work, differential protein expression in a squamous lung carcinoma cell line, DLKP, and its mitoxantrone-resistant variant (DLKP-Mitox) was investigated to elucidate other changes associated with mitoxantrone resistance. Materials and Methods: Differential protein expression between DLKP and DLKP-Mitox was investigated using 2D-DIGE technology. Proteins of interest were identified by MALDI-ToF mass spectrometry. Western blotting was used to confirm and validate some of these changes. Results: Biological variation analysis in Decyder™ software revealed a total of 343 proteins to be differentially regulated with p<0.05. Identification of 61 proteins of interest by mass spectrometry revealed changes in proteins involved in many cellular processes including apoptosis and differentiation. Conclusion: Alterations in these cellular processes and proteins present alternative sites to circumvent resistance to mitoxantrone. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved