PT  - JOURNAL ARTICLE
AU  - MURPHY, L.
AU  - CLYNES, M.
AU  - KEENAN, J.
TI  - Proteomic Analysis to Dissect Mitoxantrone Resistance-associated Proteins in a Squamous Lung Carcinoma
DP  - 2007 May 01
TA  - Anticancer Research
PG  - 1277--1284
VI  - 27
IP  - 3A
4099  - http://ar.iiarjournals.org/content/27/3A/1277.short
4100  - http://ar.iiarjournals.org/content/27/3A/1277.full
SO  - Anticancer Res2007 May 01; 27
AB  - Background: Mitoxantrone resistance has been related to the expression of a drug efflux pump breast cancer resistance pump (BCRP) but little is known of the intracellular protein changes. In this work, differential protein expression in a squamous lung carcinoma cell line, DLKP, and its mitoxantrone-resistant variant (DLKP-Mitox) was investigated to elucidate other changes associated with mitoxantrone resistance. Materials and Methods: Differential protein expression between DLKP and DLKP-Mitox was investigated using 2D-DIGE technology. Proteins of interest were identified by MALDI-ToF mass spectrometry. Western blotting was used to confirm and validate some of these changes. Results: Biological variation analysis in Decyder™ software revealed a total of 343 proteins to be differentially regulated with p<0.05. Identification of 61 proteins of interest by mass spectrometry revealed changes in proteins involved in many cellular processes including apoptosis and differentiation. Conclusion: Alterations in these cellular processes and proteins present alternative sites to circumvent resistance to mitoxantrone. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved