@article {LARSSON749, author = {D.E. LARSSON and S. GUSTAVSSON and R. HULTBORN and J. NYGREN and U. DELLE and K. ELMROTH}, title = {Chromosomal Damage in Two X-ray Irradiated Cell Lines: Influence of Cell Cycle Stage and Irradiation Temperature}, volume = {27}, number = {2}, pages = {749--753}, year = {2007}, publisher = {International Institute of Anticancer Research}, abstract = {The aim of this study was to investigate if irradiation with X-rays in different cell cycle phases resulted in a different response as measured with the micronucleus technique. In addition, the influence of irradiation temperature was investigated. Materials and Methods: Cells from a non-transformed human fibroblast cell line, HS2429, and a human breast cancer cell line, MCF-7, were synchronized by thymidine block and irradiated at either 2{\textdegree}C or 37{\textdegree}C in the G1-, S- and G2/M-phases. After cytokinesis-block by cytochalasin B, the frequency of micronuclei was determined. Results: Clear dose-response relationships were found. More micronuclei were detected in fibroblast cells irradiated in G1 and S than in G2/M, while the differences were not as prominent in MCF-7 cells. The irradiation temperature had no significant influence on the formation of micronuclei in either of the cell lines. Conclusion: The formation of micronuclei varies with the cell cycle stage at the time of irradiation. Copyright{\textcopyright} 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/27/2/749}, eprint = {https://ar.iiarjournals.org/content/27/2/749.full.pdf}, journal = {Anticancer Research} }