RT Journal Article
SR Electronic
T1 Involvement of Tiam1 in Apoptosis Induced by Bufalin in HeLa Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 245
OP 249
VO 27
IS 1A
A1 HONG CAO
A1 TOSHIKO SHIBAYAMA-IMAZU
A1 YUTAKA MASUDA
A1 TOSHIMASA SHINKI
A1 SHIGEO NAKAJO
A1 KAZUYASU NAKAYA
YR 2007
UL http://ar.iiarjournals.org/content/27/1A/245.abstract
AB Background: It has been previously demonstrated that bufalin, an active agent in the Chinese medicine chan' su, induces apoptosis in human leukemia cells by altering the expression of apoptosis-related genes, such as bcl-2 and c-myc. Tiam1 was also found to play a critical role in bufalin-induced apoptosis through the activation of the Rac1, PAK and JNK pathway in human leukemia cell lines. In the present study, the involvement of the Tiam1 gene products in bufalin-induced apoptosis in human solid tumor HeLa cells was examined. Materials and Methods: HeLa cells were treated with 10-8 M bufalin and apoptosis was measured by ELISA quantification of nucleosomes. Tiam1 mRNA levels were quantified by real-time PCR analysis and inhibited by transfected siRNA specific for Tiam1. Results: Apoptosis was induced in HeLa cells by treatment with 10-8 M bufalin. Expression of both Tiam1 mRNA and its protein was induced 0.5 h after the start of the bufalin treatment. Transfection of Tiam1-specific siRNA into HeLa cells markedly inhibited bufalin-induced apoptosis. Conclusion: Our results suggest that Tiam1 is a downstream mediator of bufalin-induced apoptosis in the human solid tumor HeLa cell line, as well as in leukemia cell lines.