PT - JOURNAL ARTICLE AU - SOTARO SADAHIRO AU - TOSHIYUKI SUZUKI AU - AKIRA TANAKA AU - KAZUTAKE OKADA AU - GOTA SAITO AU - HIROSHI MIYAKITA AU - LIN FUNG CHAN AU - YUTARO KAMEI AU - HIROSHI KAJIWARA AU - HIDEKI NAGASE TI - Induction of CD3+ and FoxP3+ T Cells in Left-sided Colorectal Tumors After UFT/LV Chemotherapy AID - 10.21873/anticanres.13310 DP - 2019 Apr 01 TA - Anticancer Research PG - 1997--2005 VI - 39 IP - 4 4099 - http://ar.iiarjournals.org/content/39/4/1997.short 4100 - http://ar.iiarjournals.org/content/39/4/1997.full SO - Anticancer Res2019 Apr 01; 39 AB - Background/Aim: Immune checkpoint inhibitors are mainly used for right-sided, microsatellite instability-high colorectal tumors. In this study, the effects of oral uracil–tegafur plus leucovorin (UFT/LV) chemotherapy on the gene expressions of four immunotherapy targets and the amounts of tumor-infiltrating lymphocytes (TILs) were investigated. Patients and Methods: Data of 260 patients with stage II or stage III colorectal cancer were analyzed. Gene expression and amount of TILs were evaluated using real-time reverse transcription polymerase chain reaction (CRT-PCR) assay and immunohistochemical staining, respectively. Results: Expression of CTLA4 and LAG3 in tumor tissues was significantly increased after UFT/LV chemotherapy, but only in left-sided tumors. The percentage of high-TIL, high-CD3 and high-FoxP3 patients in the UFT/LV group was significantly higher than that in the control group, only in left-sided tumors. Conclusion: The increase in TILs count, especially of CD3+ T cells and FoxP3+ regulatory T cells, after UFT/LV chemotherapy were specific to left-sided colorectal cancers.