RT Journal Article
SR Electronic
T1 Effect of Tissue Factor on Colorectal Cancer Stem Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2635
OP 2642
VO 38
IS 5
A1 HAMISH WILLIAM CLOUSTON
A1 PETER ADAM REES
A1 REBECCA LAMB
A1 SARAH ELIZABETH DUFF
A1 CLIONA CLARE KIRWAN
YR 2018
UL http://ar.iiarjournals.org/content/38/5/2635.abstract
AB Background/Aim: Tissue factor (TF) expression increases cancer stem cell (CSC) activity in breast and lung cancer. There are ongoing studies focused on targeting CSCs via anti-TF treatment, for breast and lung cancer therapy. Herein, the aim was to determine whether targeting TF could have an anti-CSC therapeutic role in colorectal cancer (CRC). Materials and Methods: Evaluation of colonosphere-forming efficiency (CFE) and aldehyde dehydrogenase (ALDH) expression level was used to quantify CSC activity in two CRC cell lines, after TF knockdown (TFKD) or TF over-expression (TFOE). Results: TFKD resulted in increased levels of ALDH in SW620 (1.31±0.04-fold, p<0.001) and DLD-1 (1.63±0.14-fold, p=0.04) cells. CFE was increased in SW620 (1.21±0.23% vs. 2.03±0.29%, p=0.01) and DLD-1 (0.41±0.12% vs. 0.68±0.9%, p=0.01) cells. Conversely, TFOE decreased ALDH expression (0.72±0.04-fold, p=0.001) and CFE (0.33±0.05% vs. 0.66±0.14%, p=0.006) in DLD-1, but had no impact on SW620 cells. Conclusion: In the examined CRC cell lines, TF expression was inversely related to CSC activity suggesting that anti-TF therapies may not have a role in CRC treatment.