PT  - JOURNAL ARTICLE
AU  - CHEAH, YEW HOONG
AU  - AZIMAHTOL, HAWARIAH LOPE PIHIE
AU  - ABDULLAH, NOOR RAIN
TI  - Xanthorrhizol Exhibits Antiproliferative Activity on MCF-7 Breast Cancer Cells <em>via</em> Apoptosis Induction
DP  - 2006 Nov 01
TA  - Anticancer Research
PG  - 4527--4534
VI  - 26
IP  - 6B
4099  - http://ar.iiarjournals.org/content/26/6B/4527.short
4100  - http://ar.iiarjournals.org/content/26/6B/4527.full
SO  - Anticancer Res2006 Nov 01; 26
AB  - Xanthorrhizol is a natural sesquiterpenoid compound isolated from the rhizome of Curcuma xanthorrhiza Roxb (Zingiberaceae). Xanthorrhizol was tested for a variety of important pharmacological activities including antioxidant and anti-inflammatory activities. An antiproliferation assay using the MTT method indicated that xanthorrhizol inhibited the proliferation of the human breast cancer cell line, MCF-7, with an EC50 value of 1.71 μg/ml. Three parameters including annexin-V binding assay, Hoechst 33258 staining and accumulation of sub-G1 population in DNA histogram confirmed the apoptosis induction in response to xanthorrhizol treatment. Western-blotting revealed down-regulation of the anti-apoptotic bcl-2 protein expression. However, xanthorrhizol did not affect the expression of the pro-apoptotic protein, bax, at a concentration of 1 μg/ml, 2.5 μg/ml and 5 μg/ml. The level of p53 was greatly increased, whilst PARP-1 was cleaved to 85 kDa subunits, following the treatment with xanthorrhizol at a dose-dependent manner. These results, thereby, suggest that xanthorrhizol has antiproliferative effects on MCF-7 cells by inducing apoptosis through the modulation of bcl-2, p53 and PARP-1 protein levels. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved